Experiment 1
  Preparation of Plasmid DNA
Experiment 2
  Restriction Enzyme Digestion and Agarose Gel Electrophoresis
Experiment 3
  Polymerase Chain Reaction(PCR)
Experiment 4
  DNA Purification and Construction of Recombinant DNA
Experiment 5
  Preparation of Competent E.coli Cells
Experiment 6
  Transformation of Recombinant DNA
Experiment 7
  Identification and Characterization of Positive Clones
Experiment 8
  Isolation ofTotal RNA from Animal Tissues
Experiment 9
  Molecular Hybridization
Experiment 10
  Reverse Transcription—Polymerase Chain Reaction(RT-PCR) and TA-cloning
Experiment 11
  Expression and Purification of a Fusion Protein from E.coli
Experiment 12
  Electrophoretic Mobility Gel Shift Assay(EMSA)to Detect DNA—Protein Interactions
Appendix I
  Brief Introduction to the NCBI Database
Appendix II
  Vector Information
Appendix III
  Instructions on How to Use the Equipment
References