實(shí)驗(yàn)室安全是研究者的身體健康以及教學(xué)和科研活動(dòng)順利開展的重要保障。本書采用全英文編寫，以生命科學(xué)基礎(chǔ)實(shí)驗(yàn)、綜合性自主實(shí)驗(yàn)、開放性研究實(shí)驗(yàn)以及科學(xué)研究活動(dòng)為對(duì)象，以用于基礎(chǔ)教學(xué)的一級(jí)生物安全水平基礎(chǔ)實(shí)驗(yàn)室為參照，針對(duì)實(shí)驗(yàn)過程中易發(fā)生的安全事故隱患及防范措施進(jìn)行分類陳述，圖文并茂，通俗易懂。 本書可作為高等院校相關(guān)專業(yè)留學(xué)生或雙語教學(xué)的推薦教材，也可供開展實(shí)驗(yàn)室工作的科研和管理人員參考。
★本書內(nèi)容簡(jiǎn)明實(shí)用，儀器安全操作規(guī)范除了文字?jǐn)⑹鐾�，還以操作流程圖表示；安全注意事項(xiàng)以繪圖的形式重點(diǎn)展示。通過圖文并茂和虛實(shí)互補(bǔ)，突出重點(diǎn)和細(xì)節(jié)，有很強(qiáng)的針對(duì)性和可操作性，通俗易懂。
★本書每章節(jié)后配套檢測(cè)習(xí)題，使實(shí)驗(yàn)學(xué)習(xí)更方便！
This procedure guideline was developed by Experimental Teaching Center for Life Science and Technology of Huazhong University of Science and Technology, College of Life Science and Technology to provide sufficient information on safety protection of workers and the surrounding environment in bioscience laboratory. This guideline is expected to be revised up to date guaranteeing that regulations, policies and procedures follow applicable and safe standards. All users in bioscience laboratories of educational institutions, including researchers, teachers, and students, are required to study and implement safety precautions to avoid safety accidents. This book aims to protect operators, experiment equipment and the research environment to reduce or eliminate the potential of biohazards. Neither any harms to the health of the operators nor any intentional failure of equipment is expected during bioscience experiments. Also, the research environment shall be kept fine for continuous work. Especially targeted to be applied to laboratories in general bio-laboratories for undergraduate and postgraduate education, this manual illustrates detailed procedure and guidelines for laboratory water, electricity, gas, sound, light usage and for some common apparatus. The contents are formulated on fundamental laboratory safety knowledge, standard equipment operations, the main properties of chemicals, and past laboratory experiences. The contents are concise and practical. In addition to text description, there are also standard operation procedure figures in standard operations of equipment part. Safety precautions are illustrated with figures. Therefore, this guideline is excellent with pictures and texts and are easy to understand. In addition, this guideline is scripted mainly based on Laboratory Biosafety Manual (3rd Edition, WHO), General requirements for laboratory biosafety(GB19489-2008), the Control of Substances Hazard to Health (COSHH) (http://www.hse.gov.uk/), the Globally Harmonized System of Classification and Labeling of Chemicals (GHS) etc. Moreover, this book is edited by first-line researchers in bioscience laboratories who have rich research and teaching experience, so the contents is specific and has practice guiding significance for users of bioscience laboratories.
蘇莉，女，博士，華中科技大學(xué)生命科學(xué)與技術(shù)學(xué)院教授，國(guó)jia級(jí)生命科學(xué)實(shí)驗(yàn)教學(xué)示范中心主任，主編教材多部。
Content
Chapter 1 Introduction to Basic Safety in
Bio-Laboratory 4
1.1 Introduction to biosafety levels and
general precautions in bioscience laboratory 4
1.2 Safety guidelines on water usage in
bioscience laboratory 6
1.3 Safety guidelines on electricity usage
in bioscience laboratory 8
1.4 Safety guidelines on gas usage in
bioscience laboratory 10
1.5 Safety guidelines on sound usage in
bioscience laboratory 12
1.6 Safety guidelines on light usage in
bioscience laboratory 13
Chapter 2 Standard Operations and Safety
Precautions of General Apparatus in Bio-Laboratory 16
2.1 Standard operations and safety
precautions of autoclave
usage.......................................................................................................16
2.2 Standard operations and safety
precautions of oven usage 20
2.3 Standard operations and safety
precautions of ultra-low temperature freezer 22
2.4 Standard operations and safety
precautions of liquid nitrogen container 23
2.5 Standard operations and safety
precautions of centrifuge .......................................................................................................24
2.6 Standard operations and safety
precautions of biosafety cabinet 28
2.7 Standard operations and safety
precautions of clean bench 30
2.8 Standard operations and safety precautions
of fume hood
........................................................................................................32
2.9 Standard operations and safety
precautions of UV analyzer 33
2.10 Standard operations and safety
precautions of laser 35
2.11 Standard operations and safety
precautions of laser power meter 37
2.12 Standard operation and safety
precaution of biological signal acquisition and analysis system 39
Chapter 3 General Safety Guidelines for
Common Biochemical Reagents Usages 42
3.1 Toxic reagents 42
3.2 Chemical unstable reagents 49
3.3 Animal anesthetics 53
Chapter 4 Storage and Handling Guidelines
of Experimental Biomaterials 55
4.1 Storage and handling guideline of
biomaterials in the life science laboratory 55
4.2 Blood samples of human and animal 61
4.3 Microorganism 63
4.4 Biomacromolecule 64
Chapter 5 Bio-science Safety, Emergency
Care and First Aid 66
5.1 Bio-laboratory potential fire accidents
66
5.2 Explosive Accidents 69
5.3 Poisoning and burn accidents 71
5.4 First aid medical box in bio-laboratory
75
References 76
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