第l部分 生物化學(xué)實(shí)驗(yàn)基本原理與方法
Part l Basic Principles and Methodologies of BiochemicalExperiments
I生物大分子的分離與純化的一般步驟與方法
I General Procedures and Methods for Isolation andPurification of
Biomacromolecules
11分光光度法
II Spectrophotometry
III層析
III Chromatography
IV電泳
ⅣElectr叩hOresis
V離心
V Centrifugation
V1分子生物學(xué)技術(shù)基礎(chǔ)——聚合酶鏈?zhǔn)椒磻?yīng)(PcR)

前言

第l部分  生物化學(xué)實(shí)驗(yàn)基本原理與方法

Part l Basic Principles and Methodologies of BiochemicalExperiments

  I生物大分子的分離與純化的一般步驟與方法

  I General Procedures and Methods for Isolation andPurification of

  Biomacromolecules

  11分光光度法

  II Spectrophotometry

  III層析

  III Chromatography

  IV電泳

  ⅣElectr叩hOresis

  V離心

  V Centrifugation

  V1分子生物學(xué)技術(shù)基礎(chǔ)——聚合酶鏈?zhǔn)椒磻?yīng)(PcR)

  VI Brief Introduction to Basic Technique of MolecularBiologyPolymerase

  Chain Reaction(PCR、

第2部分  基礎(chǔ)性實(shí)驗(yàn)

Part 2 Basic Experiments

  實(shí)驗(yàn)1蛋白質(zhì)和氨基酸的呈色反應(yīng)

  Experiment l  Color Reactions of Proteins and AminoAcids

  實(shí)驗(yàn)2蛋白質(zhì)的沉淀與變性

  Experiment 2 Precipitation and DenaturationofProteins

  實(shí)驗(yàn)3微量凱氏定氮法測(cè)蛋白質(zhì)總氮含量

  Experiment 3 Quantification of Crude Proteins—Kieldahl’SNitrogen

  Determination

  實(shí)驗(yàn)4雙縮脲法測(cè)定蛋白質(zhì)含量

  Experiment 4 Quantification ofProteins—Biulet Method

  實(shí)驗(yàn)5 Folin一酚試劑法測(cè)定蛋白質(zhì)含量

  Experiment 5 Quantification ofProteins———Folin—LowryMethod

  實(shí)驗(yàn)6紫外吸收法測(cè)定蛋白質(zhì)含量

  Experiment 6 Quantification ofProteins by UltravioletAbsorption

  實(shí)驗(yàn)7染料結(jié)合法測(cè)定蛋白質(zhì)含量

  Experiment 7 Quantification ofProteins by Dye—bindingMethod

  實(shí)驗(yàn)8幾種重要氨基酸(賴氨酸、色氨酸和脯氨酸)的含量測(cè)定

  Experiment 8 Determination of the Contents of SomeImportant Amino Acids

  (LysTrp and Pro)

  實(shí)驗(yàn)9血清蛋白的醋酸纖維薄膜電泳

  Experiment 9 Cellulose Acetate Membrane Electrophoresisof Serum Proteins

  實(shí)驗(yàn)10酪蛋白的制備

  Experiment 10 Preparation ofCasein

  實(shí)驗(yàn)11  SDS聚丙烯酰胺凝膠電泳(PAGE)~IJ定蛋白質(zhì)分子質(zhì)量

  Experiment 11 Molecular Weight Determination ofProteinsby SDSPAGE

  實(shí)驗(yàn)12聚丙烯酰胺凝膠等電聚焦測(cè)定蛋白質(zhì)等電點(diǎn)

  Experiment 12 pI Determination of Proteins byPolyacrylamide Gel

  Isoelectrofocusing

  實(shí)驗(yàn)13酶的特性

  Experiment 13 Properties ofEnzymes

  實(shí)驗(yàn)14多酚氧化酶米氏常數(shù)(‰)的測(cè)定

  Experiment 14 Determination of Michaelis Constant(Kin)ofPolyphenol

  Oxidase

  實(shí)驗(yàn)1 5核酸酶的活力測(cè)定

  Experiment 15 Activity Assay ofNucleases

  實(shí)驗(yàn)16谷丙轉(zhuǎn)氨酶活力測(cè)定

  Experiment 16 Determination ofActivity ofGlutamate—Pyruvate Transaminase

  實(shí)驗(yàn)l 7植物抗氧化酶類(lèi)(超氧物歧化酶、過(guò)氧化物酶和過(guò)氧化氫酶)活

  性測(cè)定

  Experiment 17 Activity Assay of Common AntioxidantEnzymes(SOD，POD

  and CAT)In Plant

  實(shí)驗(yàn)1 8乳酸脫氫酶(LDH)活性測(cè)定

  Experiment 18 Activity Assay ofLactateDehydrogenase(LDH)

  實(shí)驗(yàn)19維生素C的定量測(cè)定——2，6二氯酚靛酚法

  Experiment 19 Quantification ofVitamin C by2，6Dichlorophenolindo

  Phenol Method

  實(shí)驗(yàn)20花菜中核酸的分離和鑒定

  Experiment 20 Extraction and Identification ofNucleicAcids From

  Cauliflower

  實(shí)驗(yàn)21酵母核糖核酸的分離及組分鑒定

  Experiment 21 Extraction ofYeast RNA and IdentificationOf Its Components

  實(shí)驗(yàn)22定磷法測(cè)定核酸含量

  Experiment 22 Quantification of Nucleic Acids by Methodof Phosphate

  Determination

  實(shí)驗(yàn)23定糖法測(cè)定RNA含量

  Experiment 23  Quantification of RNA by RiboseDetermination Method

  實(shí)驗(yàn)24二苯胺法測(cè)定DNA含量

  Experiment 24 Quantification of DNA by DiphenylamineMethod

  實(shí)驗(yàn)25紫外吸收法測(cè)定核酸含量

  Experiment 25 Quantification ofNucleic Acids byUltraviolet Absorption

  Method

  實(shí)驗(yàn)26醋酸纖維薄膜電泳分離核糖核苷酸

  Experiment 26 Separation of Ribonucleotides by CelluloseAcetate Membrane

  Electrophoresis

  實(shí)驗(yàn)27  還原糖和總糖的含量測(cè)定_DNs法

  Experiment 27 Determination of Reducing Sugar and TotalSugar by DNS

  Method

  實(shí)驗(yàn)28血糖的定量測(cè)定——葡萄糖氧化酶氨基安替比林(COD—PAP)

  Experiment 28 Quantification of Blood Glucose by GlucoseOxidase4

  Aminoantipyrine(GOD—PAP)

  實(shí)驗(yàn)29粗脂肪的提取和測(cè)定

  Experiment 29 Extraction and Quantitative DeterminationofCrude Fat

  實(shí)驗(yàn)30  油脂的化學(xué)性質(zhì)

  Experiment 30 Chemical Properties ofOil or Fat

第3部分  綜合性實(shí)驗(yàn)

Part 3  Comprehensive Experiments

  實(shí)驗(yàn)3l蛋白質(zhì)的酸水解及其氨基酸的紙層析鑒定

  Experiment 3 1  Acid Hydrolysis of Proteins andIsolation and Identification

  ofAmino Acids by Paper Chromatography

  實(shí)驗(yàn)32離子交換層析分離混合氨基酸

  Experiment 32  Separating Mixed Amino Acids byIon—exchange

  Chromatography

  實(shí)驗(yàn)33血清球蛋白的鹽析分離及分子篩凝膠層析法脫鹽

  Experiment 33  Saltingout Separation of SerumGlobulins and Desalting by

  Molecular Sieving Gel Chromatography

  實(shí)驗(yàn)34糖的測(cè)定和鑒定——蒽酮試劑和紙層析法

  Experiment 34 Quantification and Identification ofSaccharides by Anthrone

  Colorimetry and Paper Chromatography

  實(shí)驗(yàn)35聚丙烯酰胺凝膠電泳分離乳酸脫氫酶同工酶

  Experiment 35  Separating LDH Isoenzymes by PAGE

  實(shí)驗(yàn)36葉綠體的制備及相關(guān)分析

  Experiment 36 Preparation and Anabrses ofChloroplasts

  實(shí)驗(yàn)37植物分子生物學(xué)系列實(shí)驗(yàn)

  Experiment 37 Series Experiments ofPlant MolecularBiology

參考文獻(xiàn)

附錄

  附錄1  基礎(chǔ)生物化學(xué)實(shí)驗(yàn)室常用儀器簡(jiǎn)介

  附錄2  常用緩沖溶液的配制